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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRLF1
Substrate solution is then added and color develops in proportion to the amount of Mouse NDUFA10
Then Streptavidin-HRP is added and binds to the Biotinylated LRAT antibody
The Rat SFRP4 (Secreted Frizzled Related Protein 4) ELISA Kit is a Sandwich enzyme immunoassay
The concentration of Horse IL12A in the samples is then determined by comparing the OD of the samples to the standard curve
Human ACO1 (Aconitase 1) ELISA Kit Packaging:48 wells The microtiter plate provided inThe Human ACO1 (Aconitase 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human ACO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACO1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
