Human 4-HNE (4-Hydroxynonenal) ELISA Kit GRN Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Mouse LRP4

C1QTNF5 present in the sample is added and binds to antibodies coated on the wells

The concentration of Human IL12Rb2 in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Rat IgG2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OxHDL

Human 4-HNE (4-Hydroxynonenal) ELISA Kit GRN Substrate solution is then addedThe Human 4 HNE (4 Hydroxynonenal) ELISA Kit measures 4 HNE in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, urine. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 4 HNE. 4 HNE in the sample or standard competes with a fixed amount of biotin labeled 4 HNE for sites on a pre coated antibody specific to 4 HNE. Free components are washed