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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa3
it binds with ABCB6 bound to precoated antibody
The OD value is proportional to the concentration of Human POSTN/OSF-2
Substrate solution is then added and color develops in proportion to the amount of Rat SHH
And then biotinylated Mouse OCLN Antibody is added and binds to OCLN in the sample
Human TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit FHC Standards or samples are addedThe Human TNF Alpha (Tumor Necrosis Factor Alpha) ELISA Kit measures TNF Alpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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