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The concentration of VEGF in the sample was calculated by drawing a standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSG
And then biotinylated Human SGSH Antibody is added and binds to SGSH in the sample
And then biotinylated Human CAMK1D Antibody is added and binds to CAMK1D in the sample
The plate has been pre-coated with Mouse CHI3L1 antibody
Human TTG (Tissue transglutaminase) ELISA Kit PPP1R50 The concentration of VEGF inThe Human TTG (Tissue transglutaminase) ELISA Kit measures tTG in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti tTG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
